Recombinant Dna Techniques Essay - 1871 Words

In this experiment, individuals were able to use recombinant DNA techniques such as cloning to alter the DNA of a certain strain of yeast called Saccharomyces Cerevisiae. By the use of cloning, scientists are able to analyze and compare the genetic material of a specific organism to another. In this lab, the recombinant DNA techniques are used to transform AmpS E. Coli to AmpR E. Coli. This was done by using an insert, a vector, and a host. The insert was S. Cerevisiae genomic DNA, E. Coli plasmid was the vector, and E. Coli was the host. The technique of gel electrophoresis was also used to determine whether the DNA was cut or not with restriction enzymes. Uncut sequences of the DNA were sent to an outside laboratory for sequencing. Then when the sequencing was complete, computer programs, such as BLAST, were used to determine whether or not the genomic insert originates from a gene and if the yeast codes for a protein that could be found within the human genome. Introduction- Sacc haromyces Cerevisiae, more commonly known as baker’s yeast or brewer’s yeast, is very favorable to use in biotechnology because of the wide range of environmental conditions it could live in. S. Cerevisiae is capable to live from freezing temperatures to about 55ââ€" ¦C, proliferate from 12ââ€" ¦C to 40ââ€" ¦C, grow within the pH range of 2.8-8.0, could be dried almost completely and still â€Å"come back alive† with the addition of water, grow and ferment at sugar concentrations of 3M, and can tolerate up to aShow MoreRelatedGenetic Engineering : The Technique Of Producing Recombinant Hybrid Dna By Combining Two Different Strands Of Dna1731 Words   |  7 Pagesthank Abdul Ahad Memon for proof reading my paper. Abstract Genetic engineering is the technique of producing recombinant hybrid DNA by combining two different strands of DNA. The fundamental concept of genetic engineering is gene cloning; the technique of altering the genetic structure of an organism. Gene cloning has four basic processes, namely cutting : the process of isolating the required starnd of DNA, modification : the process of altering the ends of the strand so that they could be attachedRead MoreDna Lab Report1345 Words   |  6 Pages The genomic DNA sample concentration of 28.5 ng/ÃŽ ¼L and its A260/A280 ratio of 1.85 indicates that the DNA was relatively pure since a 260/280 ratio of ~1.8 is generally accepted as pure for DNA (Cox, Doudna O’Donnell, 2015). The ratio of white colonies to blue colonies observed on the blue-white screen plate was 31:37, which shows that there was a greater number of E. coli DH5-ÃŽ ± that did not contain the plasmid with the foreign gene insert. Therefore, out of all of the E. coli DH5-ÃŽ ± cells thatRead MoreThe Potential Of Manipulating Genetic Engineering1571 Words   |  7 PagesThe last decades of scientific innovation centered around one technique: genetic engineering. 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